What was because your problem that led to less successful? And where did you buy your hormones and other?
I have taken two scoops of the agar, I mean the American were "pinch" per glass. Theoretically, the 45 ml jars were filled. Since I had no suitable measuring cup but, I have filled that hold roughly the same after eye. For some, the agar was also very hard for the leaves.
Before I start a new attempt, I'll buy first a suitable scoop me.
The sodium hypochlorite I have a canister with a I believe 13% solution bought, which I then 1 / 10 used diluted. How long they were in there I can not tell grad, since I'm in the Office, and my records are at home. But I think it was about 5 minutes.
In the Explanaten I chose ca 2-3 cm in length, where I have no longer used the blade tip because that was too soft for the agar.
I think you must have done already 3-4 times the whole thing to get a feel for. That is why I will try me now times across through my collection. As in recent weeks, many are driven out, I have enough young leaves to test. If my titanum then drives his third hand, I learned then hopefully enough to get more than a successful Explanat.
Has anyone here possibly else experiences with Micropropagation?
Andreas