How to : Lagerung von Pollen

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Shreck

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Montag, 21. Januar 2013, 17:26

How to : Lagerung von Pollen

Hallo,

hier wollte ich euch mal zeigen, wie ich die Pollen meiner Pflanzen lagere,wenn mal nichts zu bestäuben ist

Hier erst einmal das ganze Zubehör :

1. der passende Behälter

Diese Behälter haben ein Volumen von 25 ml. Der Behälter ist 5,5 cm hoch und 3 cm breit. 1 Behälter kostet 7 cent.
Dazu gibt es auch den passenden Halter. Da kann man 70 Behälter hineinstellen.

2. Papiertütchen für die Pollen

Diese Tütchen sind aus Pergamin. Sie werden auch von Briefmarkensammlern genutzt, um ihre Briefmarken zu sichern.
Sie sind 6 cm hoch und 4 cm breit.

3. Silica Gel ( Orange Gel) mit Farbindikator

Das Gel wird genutzt, um die Luftfeuchtigkeit in dem Behälter zu senken.

4. Aquarien Filterwatte

Diese Watte nutze ich, damit die Tüte mit den Pollen keinen direkten Kontakt zu dem Silica Gel hat.

So sieht das Ganze dann aus, wenn es fertig ist :

1 = Silica Gel
2 = Filterwatte
3 = Pergamin Tütchen mit dem Pollen

style = "font-size: 12pt" >If the pollen harvested come into the bag, then put the bag into the prepared container and lid only and thus for 24 hours in the refrigerator. The silica gel can pull any moisture from the pollen in time. After 24 hours, you can then make the bottles in the freezer compartment.

So, the pollen should be preserved.

Now that is a certainly or others think, we, I collect the pollen... It is quite simple. On the 1.Tag which (because where it stinks so) cut flower I the spathe directly at the base off, so then just have the inflorescences and the spadix are present.
Then I'll take a piece of paper and make a hole into it. Then, I prefer the paper on the spadix to the lower end of the male inflorescence. Then we have to wait only the 2nd day of flowering.
Only on this day the pollen are produced. So just wait for day 2. In the evening her scrapes then simply the pollen to down on the paper and then it fills the pollen just in such a small bag.

Small note on the edge: I can not guarantee that the pollen protrude from the storage. But many people have reported me that this method works very well. The pollination itself after 2 years in the freezer have worked great.
It is only important that the pollen is always absolutely dry stored. For this reason also the storage with silica gel.
When the thaw of the pollen should particularly well on it, that because the pollen will also dry.

I hope I have explained it quite well.

MfG
Stephan

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Mr. Titanum

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Montag, 21. Januar 2013, 20:42

Hello Stephen,

Prima Guide!
Where are the tanks?

BTW, the pollen remains dry, thawing during/after if you closed the vessel until it reaches room temperature; before opening the vessel water condense immediately and in the cold tank/pollen/silica gel...

Happy storing, Bernhard.

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Shreck

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Montag, 21. Januar 2013, 23:20

Thank you

Super tip with the defrost

I bought the containers here . 100 Containers for 7 € would be minimum

I have the post times above permanently angehoften, that it is always easy to find.

MfG
Stephan

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domi

Schüler

Beiträge: 77

Dienstag, 22. Januar 2013, 08:15

Hai

This is ne super Guide, if it times is the case for me I'll make it.

Gibts actually somewhere an area where all instructions are collected in the Forum and you can access it quickly?

MFG

Dominic

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Shreck

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Dienstag, 22. Januar 2013, 12:17

No there is not. This post is above you in the area and therefore always see.

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domi

Schüler

Beiträge: 77

Dienstag, 22. Januar 2013, 13:29

Hai

Oh great, this is ever good.

Is Fred also you, how to get to clone cultures?

Or you should collect somewhere most?

MFG

Dominic

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Shreck

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Dienstag, 22. Januar 2013, 13:38

Is also above you

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domi

Schüler

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Dienstag, 22. Januar 2013, 17:55

Super, thank you

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Seiti

Schüler

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Mittwoch, 23. Januar 2013, 17:44

Thank you

Wanted to know how long the pollen can be stored and how they are harvested and stored. Because my plants never to bloom at the same time.

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Mr. Titanum

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Donnerstag, 31. Januar 2013, 14:36

Hello Stephen,

me much yet to defrost a another detail.

If you want to prevent that are outside on the vessel condensate niederschägt, you need to fill a larger glass (E.g. a large pickle jar with twist-off ceilings) with silica gel (so 2-3 cm) and the open vessel with the pollen reins and air-tight seal with the lid.
Once the entire content has reached room / ambient temperature, you can open the glass and remove the container with the pollen without having somewhere reflected water.

As an alternative to the pickle jar a desiccator would be the more professional solution, but you can save is also the acquisition...

Regards, Bernhard.

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Shreck

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Donnerstag, 31. Januar 2013, 14:40

Also a good idea.
Is it so bad if outside reflected water? Inside, it is yes then dry, because Yes silica gel in the container.

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Mr. Titanum

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Donnerstag, 31. Januar 2013, 15:46

Hello Stephen,

Nope, bad is that non - stop just wet.
You can also wait until the vessel air is dried outside, but in a desiccator, it will stop not even wet from the outside...

Happy (pollen) storing, Bernhard

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Shreck

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Donnerstag, 31. Januar 2013, 18:17

You can dry all of course with a paper towel

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musa

Profi

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Samstag, 2. Februar 2013, 11:44

Hallo zusammen,

that sounds so pretty professional. I have ne tried simpler variant is perhaps inherently doomed to failure, but smells on the attempt.

I just rubbed the pollen with a cotton swab and the ' buds completely air-tight packaged and frozen. The amount of pollen that would I think be sufficient for ne pollination, however, I am skeptical whether the pollen survived. Read times where, that he survived only about 6 months, when it is not cooling with nitrogen (has I grad ned at hand).

How long is pollen fertile after thawing? If long enough and any ne method to work, you could about a kind of "Seed bank" under Search / offer thinking.

Love greetings

Michael

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Shreck

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Samstag, 2. Februar 2013, 11:55

Hello

your method, you would simply test, even if the pollen defrost according to the work. The method described by me works.

How long the pollen, will know exactly none. You should also test. I have gehört.dass pollen are so pretty much unlimited life, as long as they are absolutely dry.

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Shreck

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Samstag, 1. März 2014, 15:24

Hello
had Yes crispifolius frozen with this method. These have I thawed 2 days ago and so my new crispifolius flower pollinated. Apparently it worked. I'll keep you up to date.

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AGM_de

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Samstag, 1. März 2014, 17:28

Thanks for the good instructions.

My Anubias is unfortunately already withered, but at the next pint I'm testing flower that sometimes.

Two questions yet. You use Aquarium filter wool. That is something special, or would do it equally polished cotton or ordinary household cotton?

Is there a reason that the envelopes of glassine must be, or would do it also seeds bags made of paper?

Greeting
Andreas

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Shreck

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Samstag, 1. März 2014, 18:08

You can take also other material. I just had the cotton. I use it as a junction that lies the bags directly on the beads.

I take glassine bags, so sticking the pollen on the bag. If you take plastic zip bag, it could happen that the pollen stick there by electrostatic forces. And glassine should be breathable. So, air moisture can escape during the freezing process, absorbed by the silica gel.

Therefore, also became should go. Glassine is but smoother. Because the pollen not liable.

So it worked anyway, with the crispifolius pollen.

I have taken and on room temperature warm up the container from the freezer. Then I did on it and the pollen were fresh as the first day.

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Amorphophallus, einfrieren, Lagerung, Pollen, Tiefkühlfach, Tiefkühlschrank

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How to : Lagerung von Pollen

How to : Lagerung von Pollen

Hallo zusammen,

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