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1

Dienstag, 31. Juli 2007, 09:02

Meristemkultur - 'Recycling' A. scaber

Hello

somewhere there times a post (perhaps even by Mr. Titanum?) to the subject of Meristem-or in-vitro culture with a 'Guide'. Unfortunately I can no longer to find the despite hours 'googling'.

Who remembers or knows the page? : help:

Thank you!

LG,
Martina

2

Dienstag, 31. Juli 2007, 10:17

Hello Martina,.

You don't have to look in the other forum....;-)

MfG,
Bernhard.

3

Dienstag, 31. Juli 2007, 11:01

Hi Bernard,.

You give me link to another forum, also interested in:D

Greeting René

4

Dienstag, 31. Juli 2007, 11:32

Hi Bernard,.

have I already watched in other forum. I have searched and searched... Was not in Peter's Forum? Why I find this no longer:??:

But if I am now here, I make my question directly;-): somewhere in the endless expanses of www (I find now also no longer), and you can also multiply Amorphos, by manTeile of leaves and / or stem simply puts on damp substrate and sometimes wither the scrap, but including a small tuber is formed. Now yes a leaves of A. is upset scaber me. So I took the completely healthy-looking sheet and dismembering it, put it on damp substrate and waited. This is now almost a month ago, the leaf pieces are still 'original green' and there are formed because where they are in the substrate stuck little lumps... But what now? Be seen? Auxin to do this? However, what of nutrient solution stood, or?

: help:

LG,
Martina

5

Dienstag, 31. Juli 2007, 11:36

Hi René,.

This is where the exotic friends meet...

Easy to meristem (-vermehrung), in vitro or similar search. There are several contributions....

MfG,
Bernhard.

6

Dienstag, 31. Juli 2007, 11:48

Warten.... (auf Godot?)

Hello Martina,.

that listens to so exciting.

If the leaf pieces are still green after so long, I would wait for what happens.

With the addition of nutrient solution, I would be careful; hire spray with a weak fertilizer solution.
Definitely not sugary nutrient solution - because only the micro-organisms look forward...

You could use also auxin-borne Bewurzelungspulver (Rhizopon or similar).
The "lumps" could be callus (as a kind of wound tissue) easy, from which roots then form. Or it is bulbous tissue, as you already wrote.

Can you make a macro shot???

You're right, there are rumors that the proliferation of Amorphophallus through leaf cuttings/pieces, in some species to expand. I have tried but still yourself that.

Greetings,
Bernhard.

7

Dienstag, 31. Juli 2007, 12:13

Thanks for the note. Was suddenly very easy to find:D

Well, so that sterilize the and so I can override Yes now have you ever... So frankly I have not therefore expected that it could work, that is why I have made me also advance not more so really smart. : ops: the Nice hand wanted to not throw away just...

But now the ambition has packed me. Can a tuber formed for the same? But before roots must be there, or? :?? You: would recommend the use of Auxin, e.g. Superthrive? Waiting is also good - Amorphos will work with me better, experience has shown that if I let the finger of...: lach dead:

Macro is unfortunately at the moment not, but at the next opportunity, I try's times.

LG,
Martina

8

Dienstag, 31. Juli 2007, 12:48

When with the magnifying glass is the formation of a root in a piece of sheet definitely (it was not there but the day before yesterday still):D

Continued....

LG,
Martina

9

Dienstag, 31. Juli 2007, 16:11

Bin ganz aufgeregt - also gleich mal Fotos gemacht... :2thumbs:

index.php?page=Attachment&attachmentID=171 index.php?page=Attachment&attachmentID=172 index.php?page=Attachment&attachmentID=173

index.php?page=Attachment&attachmentID=174

:D(auch wenn 3 posts hintereinander nicht so gut kommen)

LG,
Martina

10

Dienstag, 31. Juli 2007, 17:13

Glückwunsch ! ! !

Hello Martina,.

: cheers: high the cups and warm Glühstrumpf! ;-)

Class photos and obviously the right choice in the substrate and the other boundary conditions.

I'm already curious whether the Saxifraga pushes even a hand before going in peace.

Good growing
Bernhard

11

Dienstag, 31. Juli 2007, 20:28

Bernhard:: cheers: great looking someone forward with me. Thank you for the annealing dies: ^ ^:

Am also very curious to see how that goes on now. Speeding tickets, roots, leaf? Certainly hope the Saxifraga grows a bit, otherwise it will be lost even in the midst of the 'huge' Pearlite grain...:D

Will wait for the time being. The substrate is a mixture of pine bark humus perlite (ups, now I have revealed my secret recipe :D ). Should therefore be inside some nutrients. And: 'never change a running system'!

LG,
Martina

12

Montag, 3. September 2007, 18:32

So this Martina, I can say only one thing: since you have had A really great idea. At that time me has been the topic of "Plant culture" in the spell. I think Bernhard knows what I mean :D but me has amazed that you it did with "normal" world. You wish much success in your small propagation. At times I will try gigas or lambii it with my: ^ ^:
Mit grünen Grüßen

Niels :D

13

Montag, 3. September 2007, 19:34

Hello

If you try it with lambii I login me ever for one.:D

I try it with konjak, because I want to maim any rare varieties. (that's that I harm do my Amorphophallu something:(:(the first time)

With kind regards
Stefan

14

Montag, 3. September 2007, 21:48

Hello Niels,.

What did Martina da, is a normal conventional horticultural propagation method that is the method of choice for some plant species: reproduction of leaf cuttings.

So, as plants and Rex Begonias be increased; can also Zamioculcas man on single leaf cuttings multiply.

The "meristem", i.e. a growth zone of undifferentiated cells, comes into play, as resulting from differentiated cells of leaf tissue again meristematische (= undifferentiated) cells must form, which is then a new sprouting plant / constitutes a new sprouting tip; then, the roots of still missing to the complete plant.

The new Meristeme may arise incidentally directly from a cell of the sheet (plants) or indirectly via a callus phase. Doing a kind of wound tissue formed by the leaf cutting (callus = bunch of undifferentiated cells) and in the callus, a new meristem forms then.

In Martina case, probably only a callus or root-like fabric originated in the then first a root plant is differentiated; to the whole plant feht in this case even the sprouting plant....

That in the Anmorphophallus is the potential for the proliferation of leaf cuttings, show bulbifer and muellerii. In other species the attempt makes kluch, uh, wisely...

By the way, I'm just not sure if it is good to write - I by people see with horror the gefledderten plants in the botanical gardens, be dismembered, which can tell the difference between my and your. In the medium term that was then likely to that interesting plants from the show houses disappear...

MfG,
Bernhard.

15

Montag, 3. September 2007, 22:35

Hello

Bernhard.... good idea. Must have time in the bot. Garden look, which as such is there...:D

No, seriously: there is obviously a risk, but this is at all plants, you can easily reproduce via cuttings Yes otherwise. So sooner or later almost no plants are expected to be issued (or only with electric fence around it). On required a good objection and you should probably about worry...

I've also added the presumption that it is only so so easy on my / was, because it is pieces of seedlings and the show perhaps in General (still) very strong growth. Can not imagine, that it is just as easy with the piece, a fully developed leaf. Or am I wrong there?

Well, the www has so much info about it, because it arrives here not really to our posts. Should real concerns is but prevail, I leave away like to continue and send you the news via pm if you are interested.

If cloning I play with the idea to try it when the stuhlmannii - so you could secure the stock a bit...

BTW: http://www.baumfarn.at/treefern/viewtopic.php?t=2019

LG,
Martina

16

Dienstag, 4. September 2007, 09:19

@ Martina
Do you see differences in the use of Clonex because?
Erwin

17

Dienstag, 4. September 2007, 15:15

«Originally posted by» "Martina"»

...
I've also added the presumption that it is only so so easy on my / was, because it is pieces of seedlings and the show perhaps in General (still) very strong growth. Can not imagine, that it is just as easy with the piece, a fully developed leaf. Or am I wrong there?...
LG,
Martina

Hello Martina,.

Since you pretend you not at all!

In the plant in vitro culture, there are already quite shine-phenomena. So, not only the age, but also sometimes the position of page piece in the leaf blade has an impact on the regeneration potential. It is sometimes important whether you put the pieces of the page with the top or bottom on the sewing medium. And so on...

To depict the development of your pieces of sheet, I think that you quietly here further can describe this in the Forum.
The Anmorphophilen here will be so in the botanical gardens and there carving up the plants. It is easier to ask the gardener, because certainly a cutting or a plant where it works better if you can offer what to Exchange.

MfG,
Bernhard.

18

Mittwoch, 5. September 2007, 17:08

«Originally posted by» "Erwin"»

@ Martina
Do you see differences in the use of Clonex because?
Erwin


Yes! And if you look closely, you will see they also;-)

Without Clonex a lumps (callus tissue) is formed first, then more from this growing 1 longer root. Immediately several roots grow with C.

For comparison:
index.php? page = attachment & attachmentID = 175

A. scaber - without C after 4 weeks
index.php? page = attachment & attachmentID = 176

A. opertus - c after 2 weeks.
The faster root growth could be but also on the light (as predicted by Bernhard)...
index.php? page = attachment & attachmentID = 178

A. scaber (not C) after 6 weeks
index.php? page = attachment & attachmentID = 177

A. scaber (treated only after 4 weeks with C) for 6 weeks

I could imagine that fails the effect in older plants / leaves even more, or it no longer works without root hormone (if possible).

Of course only time is a first impression...

LG,
Martina

P.S. thanks, Bernhard, for the statements. :^^:

19

Sonntag, 16. September 2007, 11:55

update - neue Fotos

For those who are just as curious as I - latest photos (16.9.2007 / about 11: 15 pm):

index.php? page = attachment & attachmentID = 181 index.php? page = attachment & attachmentID = 180 index.php? page = attachment & attachmentID = 179

There will probably whether these lumps now be speeding tickets or it's only to callus tissue show soon. Should it be speeding tickets, then the piece treated with Clonex is arg to the detriment - roots has made only a pile. Or the handle pieces off is 'poor'...

The vessel is still 30-40 cm from the window with moderate light but not direct sun. Today the first time given a tiny amount of liquid fertilizer in the jets of water.

LG,
Martina

20

Sonntag, 16. September 2007, 20:40

Hello Martina,.

on the last of the three images clearly a small tuber is in my opinion, the two beautiful roots grow out.
Time will tell whether even a sprouting plant that then forms a leaf bud, it is justified...

I would be quite confident.

Happy budding,
Bernhard.

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